 | Oleh RymarenkoSequence space and properties of XPO1-dependent nuclear export signals |
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| ISBN: | 978-3-8440-7909-8 |
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| Reihe: | Biochemie |
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| Schlagwörter: | Nuclear transport; Nuclear; nuclear export |
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| Publikationsart: | Dissertation |
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| Sprache: | Englisch |
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| Seiten: | 168 Seiten |
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| Abbildungen: | 39 Abbildungen |
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| Gewicht: | 249 g |
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| Format: | 21 x 14,8 cm |
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| Bindung: | Paperback |
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| Preis: | 48,80 € / 61,10 SFr |
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| Erscheinungsdatum: | März 2021 |
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| Zusammenfassung | Exportin 1 (XPO1) mediates one of the most versatile protein transport pathways in the cell, exporting hundreds of different proteins from the nucleus to the cytoplasm. In this dissertation, I employ filamentous phage display coupled with high-throuput DNA sequencing to establish the sequence space of the most common type the nuclear export signals (NESes) recognized by XPO1. I confirm the role of the discovered sequence features in determining the nuclear export efficiency in the cell. I show the utility of the obtained information in engineering of NES-like sequences, including creating artificial NESes or NES-like sequences that bind XPO1 with high affinity. Additionally, I explore the use of the established sequence space parameters for prediction of NESes based on a protein sequence. This thesis could be of interest to biochemists and cell biologists interested in protein localization in the cell as well as specialists in protein-peptide interactions. |
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