Shop : Details
Shop
Details
Marvin Kadisch
Stabilizing whole-cell biocatalysts
En route to more efficient fatty acid methyl ester bioprocessing
Whole-cell biocatalysts offer great potential for the development of sustainable processes that are difficult to achieve chemically and can replace or complement traditional chemical and pharmaceutical processes. However, microbial cells are not evolved for the production of industrially-relevant compounds, which often exert toxicity and therefore destabilize wholecell biocatalysts. In order to overcome limitations related to stability and to exploit the full potential of whole-cell biocatalysts, a concerted strategy combining pathway, cellular, reaction, and process engineering has to be pursued.
In this thesis, a microbial oxyfunctionalization reaction was selected as a model bioprocess system to understand the mechanisms that affect the stability of whole-cell biocatalysts in a bioprocess and to exemplarily show selective engineering approaches to overcome such stability influencing factors. Respective biocatalysts were based on genetically modified E. coli containing the alkane monooxygenase system AlkBGT and the outer membrane protein AlkL from P. putida GPo1, enabling oxyfunctionalization of renewable fatty acid methyl esters (FAMEs).
In this thesis, a microbial oxyfunctionalization reaction was selected as a model bioprocess system to understand the mechanisms that affect the stability of whole-cell biocatalysts in a bioprocess and to exemplarily show selective engineering approaches to overcome such stability influencing factors. Respective biocatalysts were based on genetically modified E. coli containing the alkane monooxygenase system AlkBGT and the outer membrane protein AlkL from P. putida GPo1, enabling oxyfunctionalization of renewable fatty acid methyl esters (FAMEs).
Schlagwörter: stability; whole-cell biocatalysis; monooxygenase; AlkBGT; FAMEs
Verfügbare Online-Dokumente zu diesem Titel
Sie benötigen den Adobe Reader, um diese Dateien ansehen zu können. Hier erhalten Sie eine kleine Hilfe und Informationen, zum Download der PDF-Dateien.
Bitte beachten Sie, dass die Online-Dokumente nicht ausdruckbar und nicht editierbar sind.
Bitte beachten Sie auch weitere Informationen unter: Hilfe und Informationen.
Bitte beachten Sie auch weitere Informationen unter: Hilfe und Informationen.
| Dokument |  | Gesamtdokument | ||
| Dateiart |  | |||
| Kosten |  | 37,35 € | ||
| Aktion |  | Zahlungspflichtig kaufen und download der Datei | ||
| Dokument |  | Inhaltsverzeichnis | ||
| Dateiart | | |||
| Kosten |  | frei | ||
| Aktion |  | Download der Datei | ||
Benutzereinstellungen für registrierte Online-Kunden (Online-Dokumente)
Sie können hier Ihre Adressdaten ändern sowie bereits georderte Dokumente erneut aufrufen.
Benutzer
Nicht angemeldet
Export bibliographischer Daten
Shaker Verlag GmbHAm Langen Graben 15a52353 Düren
Mo. - Do. 8:00 Uhr bis 16:00 UhrFr. 8:00 Uhr bis 15:00 Uhr
Kontaktieren Sie uns. Wir helfen Ihnen gerne weiter.
