Shop : Details
Shop
Details
45,80 €ISBN 978-3-8440-5496-5Paperback148 Seiten42 Abbildungen222 g21 x 14,8 cmEnglischDissertation
September 2017
Cornelis Lütgebaucks
Lipid membrane characterization with second harmonic scattering: surface potentials, ionization, membrane asymmetry and hydration
Membranes, composed of a variety of lipids and other biomolecules, mediate signaling processes between cells and their aqueous environment. To fulfill this function, membranes can vary their composition leaflet-specific and thus alter their surface properties. To fully understand the impact of these processes on the molecular level, it is necessary to develop tools that can access the molecular properties of free-floating model membranes label-free. These tools are ideally surface-specific.
In this thesis, we apply the nonlinear optical techniques second harmonic scattering (SHS) and vibrational sum-frequency scattering (SFS) together with electrokinetic measurements to label-free characterize the interfacial properties, hydration structure and surface potentials of liposomes in aqueous solutions.
We generalize the nonlinear optical theory to describe the second-order surface response from interfaces with aqueous solutions independent of the ionic strength for reflection, transmission and scattering geometries. Then, we apply this theory to demonstrate that SHS patterns of liposomes and oil droplets contain all necessary information to extract the absolute surface potential of the respective particles without assuming a model for the interfacial structure but analyzing the hydration structure of the interface. Subsequently we quantify hydration and lipid asymmetries in binary mixed lipid membranes using SHS and SFS. Finally, we quantify the surface properties of phosphocholine and phosphoserine containing membranes and analyze the counterion condensation.
The work of this thesis demonstrates that biotechnological studies but also very fundamental questions can be equally well adressed with these nonlinear optical techniques.
In this thesis, we apply the nonlinear optical techniques second harmonic scattering (SHS) and vibrational sum-frequency scattering (SFS) together with electrokinetic measurements to label-free characterize the interfacial properties, hydration structure and surface potentials of liposomes in aqueous solutions.
We generalize the nonlinear optical theory to describe the second-order surface response from interfaces with aqueous solutions independent of the ionic strength for reflection, transmission and scattering geometries. Then, we apply this theory to demonstrate that SHS patterns of liposomes and oil droplets contain all necessary information to extract the absolute surface potential of the respective particles without assuming a model for the interfacial structure but analyzing the hydration structure of the interface. Subsequently we quantify hydration and lipid asymmetries in binary mixed lipid membranes using SHS and SFS. Finally, we quantify the surface properties of phosphocholine and phosphoserine containing membranes and analyze the counterion condensation.
The work of this thesis demonstrates that biotechnological studies but also very fundamental questions can be equally well adressed with these nonlinear optical techniques.
Schlagwörter: second harmonic scattering; nonlinear optics; membranes; surface potentials
Verfügbare Online-Dokumente zu diesem Titel
DOI 10.2370/9783844054965
Sie benötigen den Adobe Reader, um diese Dateien ansehen zu können. Hier erhalten Sie eine kleine Hilfe und Informationen, zum Download der PDF-Dateien.
Bitte beachten Sie, dass die Online-Dokumente nicht ausdruckbar und nicht editierbar sind.
Bitte beachten Sie auch weitere Informationen unter: Hilfe und Informationen.
Bitte beachten Sie auch weitere Informationen unter: Hilfe und Informationen.
| Dokument |  | Gesamtdokument | ||
| Dateiart |  | |||
| Kosten |  | 34,35 € | ||
| Aktion |  | Zahlungspflichtig kaufen und download der Datei | ||
| Dokument |  | Inhaltsverzeichnis | ||
| Dateiart | | |||
| Kosten |  | frei | ||
| Aktion |  | Download der Datei | ||
Benutzereinstellungen für registrierte Online-Kunden (Online-Dokumente)
Sie können hier Ihre Adressdaten ändern sowie bereits georderte Dokumente erneut aufrufen.
Benutzer
Nicht angemeldet
Export bibliographischer Daten
Shaker Verlag GmbH
Am Langen Graben 15a
52353 Düren
Am Langen Graben 15a
52353 Düren
Mo. - Do. 8:00 Uhr bis 16:00 Uhr
Fr. 8:00 Uhr bis 15:00 Uhr
Fr. 8:00 Uhr bis 15:00 Uhr
Kontaktieren Sie uns. Wir helfen Ihnen gerne weiter.
